| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 231 | Holdemania filiformis | TCACGCTGTTCAACCGCA | TTGACATGCACGAGCGGA | 60.20 | 59.66 | 235 | 76 |
100.00%
|
50.00%
|
| Primer 232 | Holdemania filiformis | GCCAACGGAGCGGAAATTC | TGAGCCATCGACATCGCA | 59.86 | 59.11 | 245 | 76 |
100.00%
|
50.00%
|
| Primer 233 | Holdemania filiformis | CGCACCCTGCAGCTGTTAA | TTCCGGTTGGCCATGCAT | 60.97 | 59.96 | 195 | 76 |
100.00%
|
50.00%
|
| Primer 234 | Holdemania filiformis | GCGTTCAGGAAAGCGGTGT | CCGCTGACAAAAGACGACT | 61.25 | 58.10 | 173 | 76 |
100.00%
|
50.00%
|
| Primer 235 | Holdemania filiformis | GCGTTCAGGAAAGCGGTGT | ATCCGCTGACAAAAGACGACT | 61.25 | 60.00 | 175 | 76 |
100.00%
|
50.00%
|
| Primer 236 | Holdemania filiformis | TTATGTCGGCAGCGGAGT | CGCGACGTCAAACTTGCA | 58.71 | 59.38 | 163 | 76 |
100.00%
|
50.00%
|
| Primer 237 | Holdemania filiformis | GGCCAAAACCGCAAGCAA | ATCCACAGCCTGAACGCTC | 59.89 | 60.08 | 196 | 76 |
100.00%
|
50.00%
|
| Primer 238 | Holdemania filiformis | GGCCAAAACCGCAAGCAA | TTTACGCGTCGTCCTGACC | 59.89 | 60.08 | 268 | 76 |
100.00%
|
50.00%
|
| Primer 239 | Holdemania filiformis | TTCCGTTGTCGCTGCTGT | TCCGCTCGTAAAACGGCA | 59.89 | 59.66 | 192 | 76 |
100.00%
|
50.00%
|
| Primer 240 | Holdemania filiformis | ACTGGTGGCCTAAGCTCCT | TGTCAATCGCAGCCTGGA | 60.23 | 58.93 | 201 | 76 |
100.00%
|
50.00%
|